Tracking endocytosis and intracellular distribution of spherical nucleic acids with correlative single-cell imaging

Mengmeng Liu, Fei Wang, Xueli Zhang, Xiuhai Mao, Lihua Wang, Yang Tian, Chunhai Fan, Qian Li

Published: 2020-12-06 DOI: 10.1038/s41596-020-00420-1

spherical nucleic acids

correlative imaging

plasmonic nanoparticles

endocytosis

intracellular distribution

AI 解读

Efficient and safe single-cell cloning of human pluripotent stem cells using the CEPT cocktail

Profiling native pulmonary basement membrane stiffness using atomic force microscopy

Genetic circuit design automation with Cello 2.0

Synthesis of siRNA nanoparticles to silence plaque-destabilizing gene in atherosclerotic lesional macrophages

Scalable dual-omics profiling with single-nucleus chromatin accessibility and mRNA expression sequencing 2 (SNARE-seq2)

A practical guide to scanning light-field microscopy with digital adaptive optics

Effective high-throughput isolation of fully human antibodies targeting infectious pathogens

Improved sampling and DNA extraction procedures for microbiome analysis in food-processing environments

MOF-derived nanoporous carbons with diverse tunable nanoarchitectures

Tutorial: a statistical genetics guide to identifying HLA alleles driving complex disease

查看全部

Sections

Figures

References

A comprehensive understanding of interactions between nanoparticles (NPs) and biological components is critical to the clinical application of NPs and nanomedicine. Here we provide a step-by-step correlative imaging approach to investigate plasmonic NPs o
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
References
ACKNOWLEDGMENTS

Cui, Z. et al. Nanodiamond autophagy inhibitor allosterically improves the arsenical-based therapy of solid tumors. Nat. Commun. 9, 4347 (2018).
Jiang, D. W. et al. DNA origami nanostructures can exhibit preferential renal uptake and alleviate acute kidney injury. Nat. Biomed. Eng. 2, 865–877 (2018).
Shi, B. et al. Theranostic nanoplatform with hydrogen sulfide activatable NIR responsiveness for imaging-guided on-demand drug release. Angew. Chem. Int. Ed. Engl. 58, 16826–16830 (2019).
Tang, W. et al. Emerging blood-brain-barrier-crossing nanotechnology for brain cancer theranostics. Chem. Soc. Rev. 48, 2967–3014 (2019).
Yu, G. C. et al. A discrete organoplatinum(II) metallacage as a multimodality theranostic platform for cancer photochemotherapy. Nat. Commun. 9, 4335 (2018).
Yamankurt, G. et al. Exploration of the nanomedicine-design space with high-throughput screening and machine learning. Nat. Biomed. Eng. 3, 318–327 (2019).
Ioannidis, J. P. A., Kim, B. Y. S. & Trounson, A. How to design preclinical studies in nanomedicine and cell therapy to maximize the prospects of clinical translation. Nat. Biomed. Eng. 2, 797–809 (2018).
van der Meel, R. et al. Smart cancer nanomedicine. Nat. Nanotechnol. 14, 1007–1017 (2019).
Chinen, A. B., Guan, C. X. M., Ko, C. H. & Mirkin, C. A. The impact of protein corona formation on the macrophage cellular uptake and biodistribution of spherical nucleic acids. Small 13, 1603847 (2017).
Li, H. et al. Molecular spherical nucleic acids. Proc. Natl Acad. Sci. USA 115, 4340–4344 (2018).
Rees, P., Wills, J. W., Brown, M. R., Barnes, C. M. & Summers, H. D. The origin of heterogeneous nanoparticle uptake by cells. Nat. Commun. 10, 2341 (2019).
Hinde, E. et al. Pair correlation rnicroscopy reveals the role of nanoparticle shape in intracellular transport and site of drug release. Nat. Nanotechnol. 12, 81–89 (2017).
Huang, Z. N., Cole, L. E., Callmann, C. E., Wang, S. & Mirkin, C. A. Sequence multiplicity within spherical nucleic acids. ACS Nano 14, 1084–1092 (2020).
Liu, M. et al. Real-time visualization of clustering and intracellular transport of gold nanoparticles by correlative imaging. Nat. Commun. 8, 15646 (2017).
Behzadi, S. et al. Cellular uptake of nanoparticles: journey inside the cell. Chem. Soc. Rev. 46, 4218–4244 (2017).
Xie, X. et al. Unraveling cell-type-specific targeted delivery of membrane-camouflaged nanoparticles with plasmonic imaging. Nano Lett. 20, 5228–5235 (2020).
Shen, J. et al. Fractal nanoplasmonic labels for supermultiplex imaging in single cells. J. Am. Chem. Soc. 141, 11938–11946 (2019).
Borkowska, M. et al. Targeted crystallization of mixed-charge nanoparticles in lysosomes induces selective death of cancer cells. Nat. Nanotechnol. 15, 331–341 (2020).
Wu, X. C. A., Choi, C. H. J., Zhang, C., Hao, L. L. & Mirkin, C. A. Intracellular fate of spherical nucleic acid nanoparticle conjugates. J. Am. Chem. Soc. 136, 7726–7733 (2014).
Narayan, S. P. et al. The sequence-specific cellular uptake of spherical nucleic acid nanoparticle conjugates. Small 11, 4173–4182 (2015).
Skakuj, K. et al. Conjugation chemistry-dependent T-cell activation with spherical nucleic acids. J. Am. Chem. Soc. 140, 1227–1230 (2018).
Sita, T. L. et al. Dual bioluminescence and near-infrared fluorescence monitoring to evaluate spherical nucleic acid nanoconjugate activity in vivo. Proc. Natl Acad. Sci. USA 114, 4129–4134 (2017).
Choi, C. H. J., Hao, L., Narayan, S. P., Auyeung, E. & Mirkin, C. A. Mechanism for the endocytosis of spherical nucleic acid nanoparticle conjugates. Proc. Natl Acad. Sci. USA 110, 7625–7630 (2013).
Li, K. et al. DNA-directed assembly of gold nanohalo for quantitative plasmonic imaging of single-particle catalysis. J. Am. Chem. Soc. 137, 4292–4295 (2015).
Liu, M. M. et al. Automated nanoplasmonic analysis of spherical nucleic acids clusters in single cells. Anal. Chem. 92, 1333–1339 (2020).
Jiang, Y. et al. Aptamer/AuNP biosensor for colorimetric profiling of exosomal proteins. Angew. Chem. Int. Ed. Engl. 56, 11916–11920 (2017).
Lee, K., Cui, Y., Lee, L. P. & Irudayaraj, J. Quantitative imaging of single mRNA splice variants in living cells. Nat. Nanotechnol. 9, 474–480 (2014).
Grigolato, F., Colonabo, C., Ferrari, R., Rezabkova, L. & Arosio, P. Mechanistic origin of the combined effect of surfaces and mechanical agitation on amyloid formation. ACS Nano 11, 11358–11367 (2017).
Dominguez-Medina, S. et al. Adsorption and unfolding of a single protein triggers nanoparticle aggregation. ACS Nano 10, 2103–2112 (2016).
Xu, F. et al. Lipid-mediated targeting with membrane-wrapped nanoparticles in the presence of corona formation. ACS Nano 10, 1189–1200 (2016).
Sugikawa, K., Kadota, T., Yasuhara, K. & Ikeda, A. Anisotropic self-assembly of citrate-coated gold nanoparticles on fluidic liposomes. Angew. Chem. 128, 4127–4131 (2016).
Yu, X. et al. Dressing up nanoparticles: a membrane wrap to induce formation of the virological synapse. ACS Nano 9, 4182–4192 (2015).
Choi, C. K. K. et al. Dopamine-mediated assembly of citrate-capped plasmonic nanoparticles into stable core-shell nanoworms for intracellular applications. ACS Nano 13, 5864–5884 (2019).
Sindhwani, S. et al. The entry of nanoparticles into solid tumours. Nat. Mater. 19, 566–575 (2020).
Cheng, X. J. et al. Light-triggered assembly of gold nanoparticles for photothermal therapy and photoacoustic imaging of tumors in vivo. Adv. Mater. 29, 1604894 (2017).
Dai, Q. et al. Quantifying the ligand-coated nanoparticle delivery to cancer cells in solid tumors. ACS Nano 12, 8423–8435 (2018).
Tan, S. F. et al. Real-time imaging of the formation of Au–Ag core-shell nanoparticles. J. Am. Chem. Soc. 138, 5190–5193 (2016).
Morimura, H. et al. Nano-analysis of DNA conformation changes induced by transcription factor complex binding using plasmonic nanodimers. ACS Nano 7, 10733–10740 (2013).
Visser, E. W. A., Horaacek, M. & Zijlistra, P. Plasmon rulers as a probe for real-time microsecond conformational dynamics of single molecules. Nano Lett. 18, 7927–7934 (2018).
Readman, C. et al. Anomalously large spectral shifts near the quantum tunnelling limit in plasmonic rulers with subatomic resolution. Nano Lett. 19, 2051–2058 (2019).
Chen, W., Zhang, S. P., Deng, Q. & Xu, H. X. Probing of sub-picometer vertical differential resolutions using cavity plasmons. Nat. Commun. 9, 801 (2018).
Syed, A. M. et al. Three-dimensional imaging of transparent tissues via metal nanoparticle labeling. J. Am. Chem. Soc. 139, 9961–9971 (2017).
Wang, K. et al. In situ detection and imaging of telomerase activity in cancer cell lines via disassembly of plasmonic core–satellites nanostructured probe. Anal. Chem. 89, 7262–7268 (2017).
Wu, D., Chen, Y., Hou, S., Fang, W. & Duan, H. Intracellular and cellular detection by SERS-active plasmonic nanostructures. Chembiochem 20, 2432–2441 (2019).
Nam, W. et al. Refractive-index-insensitive nanolaminated SERS substrates for label-free Raman profiling and classification of living cancer cells. Nano Lett 19, 7273–7281 (2019).
Zhang, Z. Q. et al. Quantitative evaluation of surface-enhanced Raman scattering nanoparticles for intracellular pH sensing at a single particle level. Anal. Chem. 91, 3254–3262 (2019).
Ando, J., Fujita, K., Smith, N. I. & Kawata, S. Dynamic SERS imaging of cellular transport pathways with endocytosed gold nanoparticles. Nano Lett. 11, 5344–5348 (2011).
Mayer, K. M. & Hafner, J. H. Localized surface plasmon resonance sensors. Chem. Rev. 111, 3828–3857 (2011).
Chen, Y., Xianyu, Y. & Jiang, X. Surface modification of gold nanoparticles with small molecules for biochemical analysis. Acc. Chem. Res. 50, 310–319 (2017).
Ye, S., Li, X., Wang, M. & Tang, B. Fluorescence and SERS imaging for the simultaneous absolute quantification of multiple miRNAs in living cells. Anal. Chem. 89, 5124–5130 (2017).
Dong, B. et al. Reversible self-assembly of nanoprobes in live cells for dynamic intracellular pH imaging. ACS Nano 13, 1421–1432 (2019).
Halo, T. L. et al. NanoFlares for the detection, isolation, and culture of live tumor cells from human blood. Proc. Natl Acad. Sci. USA 111, 17104–17109 (2014).
Wu, Y. et al. Gold nanoparticle loaded split-DNAzyme probe for amplified miRNA detection in living cells. Anal. Chem. 89, 8377–8383 (2017).
Li, D., Zhou, W., Yuan, R. & Xiang, Y. A DNA-fueled and catalytic molecule machine lights up trace under-expressed microRNAs in living cells. Anal. Chem. 89, 9934–9940 (2017).
Carnevale, K. J. F., Riskowski, R. A. & Strouse, G. F. A gold nanoparticle bio-optical transponder to dynamically monitor intracellular pH. ACS Nano 12, 5956–5968 (2018).
Yang, Y. et al. FRET nanoflares for intracellular mRNA detection: avoiding false positive signals and minimizing effects of system fluctuations. J. Am. Chem. Soc. 137, 8340–8343 (2015).
Bastús, N. G., Comenge, J. & Puntes, V. Kinetically controlled seeded growth synthesis of citrate-stabilized gold nanoparticles of up to 200 nm: size focusing versus Ostwald ripening. Langmuir 27, 11098–11105 (2011).
Schindelin, J. et al. Fiji: an open-source platform for biological-image analysis. Nat Methods 9, 676–682 (2012).
Cutler, J. I., Auyeung, E. & Mirkin, C. A. Spherical nucleic acids. J. Am. Chem. Soc. 134, 1376–1391 (2012).
Zhang, X., Servos, M. R. & Liu, J. Instantaneous and quantitative functionalization of gold nanoparticles with thiolated DNA using a pH-assisted and surfactant-free route. J. Am. Chem. Soc. 134, 7266–7269 (2012).
Liu, B. & Liu, J. Freezing directed construction of bio/nano interfaces: reagentless conjugation, denser spherical nucleic acids, and better nanoflares. J. Am. Chem. Soc. 139, 9471–9474 (2017).
Lee, H. E. et al. Amino-acid- and peptide-directed synthesis of chiral plasmonic gold nanoparticles. Nature 556, 360–365 (2018).
Hakala, T. K. et al. Lasing in dark and bright modes of a finite-sized plasmonic lattice. Nat. Commun. 8, 13687 (2017).
Fang, W. et al. Quantizing single-molecule surface-enhanced Raman scattering with DNA origami metamolecules. Sci. Adv. 5, eaau4506 (2019).

Tracking endocytosis and intracellular distribution of spherical nucleic acids with correlative single-cell imaging

Supplementary information

Supplementary Information

Reporting Summary

Supplementary Software 1

Supplementary Data 1

Supplementary Data 2

Supplementary Data 3

推荐阅读