Isotropic imaging across spatial scales with axially swept light-sheet microscopy

Joerg Bewersdorf, Fabian F. Voigt, Kevin M. Dean, Tonmoy Chakraborty, Stephan Daetwyler, Jinlong Lin, Gerard Garrelts, Ons M’Saad, Hannahmariam T. Mekbib, Martina Schaettin, Esther T. Stoeckli, Fritjof Helmchen, Reto Fiolka

Published: 2022-07-12 DOI: 10.1038/s41596-022-00706-6

Light-sheet fluorescence microscopy

Axially swept light-sheet microscopy

Optical resolution

Molecular detail imaging

Instrument control software

AI 解读

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Light-sheet fluorescence microscopy is a rapidly growing technique that has gained tremendous popularity in the life sciences owing to its high-spatiotemporal resolution and gentle, non-phototoxic illumination. In this protocol, we provide detailed direct
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
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References
ACKNOWLEDGMENTS

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Isotropic imaging across spatial scales with axially swept light-sheet microscopy

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